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pbluescript vectors  (New England Biolabs)


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    Structured Review

    New England Biolabs pbluescript vectors

    Pbluescript Vectors, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 6935 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbluescript+vector/XbaI/bio_rxiv__2025__11__18__688292-201-8-18
    Average 99 stars, based on 6935 article reviews
    pbluescript vectors - by Bioz Stars, 2026-10
    99/100 stars

    Images

    1) Product Images from "CANVAS causing AAGGG repeat expansions cause tissue-specific reduction in RFC1 expression and increase sensitivity to DNA damage"

    Article Title: CANVAS causing AAGGG repeat expansions cause tissue-specific reduction in RFC1 expression and increase sensitivity to DNA damage

    Journal: bioRxiv

    doi: 10.1101/2025.11.18.688292


    Figure Legend Snippet:

    Techniques Used: Luciferase

    Related Articles

    Plasmid Preparation:

    Article Title: Bovine CD18 Is Necessary and Sufficient To Mediate Mannheimia ( Pasteurella ) haemolytica Leukotoxin-Induced Cytolysis
    Article Snippet: .. In this study, the cDNA for bovine CD18 was released from pBluescript vector by using Acc I (New England Biolabs, Beverly, Mass.). .. The resultant DNA fragment was subcloned into the Acc I restriction site in the multiple cloning site of eukaryotic expression vector pCI-neo (Promega, Madison, Wis.) to yield the expression vector pMD-1 (Fig. ).

    Article Title: The minimal intrinsic stochasticity of constitutively expressed eukaryotic genes is sub-Poissonian
    Article Snippet: Either the FirstChoice RLM-RACE kit with SuperScript IV (Invitrogen) or the GeneRacer kit (Invitrogen L150201) with SuperScript III (Invitrogen) was used to prepare the cDNA for 5’ UTR sequencing. cDNA was amplified with two rounds of nested PCR targeting the gene of interest. .. The PCR product was inserted into a pBlueScript vector using Gibson assembly (NEB, E2611) and transformed into NEB 5-alpha competent E. coli (NEB, C2992). ..

    Article Title: A novel GMMA-based gonococcal vaccine demonstrates functional immune responses in mice
    Article Snippet: .. PCR products were purified using the kit Wizard SV Gel and PCR Clean-Up System (Promega), according to the manufacturer’s instructions, and cloned as XbaI-SmaI (UP rmp ), SmaI (eryR) and SmaI-XhoI (DOWN rmp ) into a pBluescript vector digested with XbaI-XhoI (NEB). .. Amplification of upstream and downstream regions of the lgtF gene was performed with the primer couples lgtF-UP-fw / rv and lgtF-DO-fw / rv , respectively, using 50 ng of genomic DNA as template; the amplification of the chloramphenicol resistance gene ( cmR ) was done with the primer couple cloKOF / R using 10 ng of a synthetic DNA template (Geneart).

    Purification:

    Article Title: The thymidylate synthase gene of Hz-1 virus: a gene captured from its lepidopteran host.
    Article Snippet: The sequence analysis of a thymidylate synthase gene was identified in the Hz-1 virus Hin dIII-D fragment.. The viral thymidylate synthase gene encodes a protein of 295 amino acids, and is closely related to that of insect, mammals and herpesvirus.. The thymidylate synthase gene identified was a genuine viral gene in that it was only detected in cells infected with Hz-1 virus but not in the mock infected cells, by Southern blot analysis and by RT-PCR.

    Article Title: A novel GMMA-based gonococcal vaccine demonstrates functional immune responses in mice
    Article Snippet: .. PCR products were purified using the kit Wizard SV Gel and PCR Clean-Up System (Promega), according to the manufacturer’s instructions, and cloned as XbaI-SmaI (UP rmp ), SmaI (eryR) and SmaI-XhoI (DOWN rmp ) into a pBluescript vector digested with XbaI-XhoI (NEB). .. Amplification of upstream and downstream regions of the lgtF gene was performed with the primer couples lgtF-UP-fw / rv and lgtF-DO-fw / rv , respectively, using 50 ng of genomic DNA as template; the amplification of the chloramphenicol resistance gene ( cmR ) was done with the primer couple cloKOF / R using 10 ng of a synthetic DNA template (Geneart).

    Agarose Gel Electrophoresis:

    Article Title: The thymidylate synthase gene of Hz-1 virus: a gene captured from its lepidopteran host.
    Article Snippet: The sequence analysis of a thymidylate synthase gene was identified in the Hz-1 virus Hin dIII-D fragment.. The viral thymidylate synthase gene encodes a protein of 295 amino acids, and is closely related to that of insect, mammals and herpesvirus.. The thymidylate synthase gene identified was a genuine viral gene in that it was only detected in cells infected with Hz-1 virus but not in the mock infected cells, by Southern blot analysis and by RT-PCR.

    Transformation Assay:

    Article Title: The thymidylate synthase gene of Hz-1 virus: a gene captured from its lepidopteran host.
    Article Snippet: The sequence analysis of a thymidylate synthase gene was identified in the Hz-1 virus Hin dIII-D fragment.. The viral thymidylate synthase gene encodes a protein of 295 amino acids, and is closely related to that of insect, mammals and herpesvirus.. The thymidylate synthase gene identified was a genuine viral gene in that it was only detected in cells infected with Hz-1 virus but not in the mock infected cells, by Southern blot analysis and by RT-PCR.

    Article Title: The minimal intrinsic stochasticity of constitutively expressed eukaryotic genes is sub-Poissonian
    Article Snippet: Either the FirstChoice RLM-RACE kit with SuperScript IV (Invitrogen) or the GeneRacer kit (Invitrogen L150201) with SuperScript III (Invitrogen) was used to prepare the cDNA for 5’ UTR sequencing. cDNA was amplified with two rounds of nested PCR targeting the gene of interest. .. The PCR product was inserted into a pBlueScript vector using Gibson assembly (NEB, E2611) and transformed into NEB 5-alpha competent E. coli (NEB, C2992). ..

    Polymerase Chain Reaction:

    Article Title: The minimal intrinsic stochasticity of constitutively expressed eukaryotic genes is sub-Poissonian
    Article Snippet: Either the FirstChoice RLM-RACE kit with SuperScript IV (Invitrogen) or the GeneRacer kit (Invitrogen L150201) with SuperScript III (Invitrogen) was used to prepare the cDNA for 5’ UTR sequencing. cDNA was amplified with two rounds of nested PCR targeting the gene of interest. .. The PCR product was inserted into a pBlueScript vector using Gibson assembly (NEB, E2611) and transformed into NEB 5-alpha competent E. coli (NEB, C2992). ..

    Article Title: A novel GMMA-based gonococcal vaccine demonstrates functional immune responses in mice
    Article Snippet: .. PCR products were purified using the kit Wizard SV Gel and PCR Clean-Up System (Promega), according to the manufacturer’s instructions, and cloned as XbaI-SmaI (UP rmp ), SmaI (eryR) and SmaI-XhoI (DOWN rmp ) into a pBluescript vector digested with XbaI-XhoI (NEB). .. Amplification of upstream and downstream regions of the lgtF gene was performed with the primer couples lgtF-UP-fw / rv and lgtF-DO-fw / rv , respectively, using 50 ng of genomic DNA as template; the amplification of the chloramphenicol resistance gene ( cmR ) was done with the primer couple cloKOF / R using 10 ng of a synthetic DNA template (Geneart).

    Clone Assay:

    Article Title: A novel GMMA-based gonococcal vaccine demonstrates functional immune responses in mice
    Article Snippet: .. PCR products were purified using the kit Wizard SV Gel and PCR Clean-Up System (Promega), according to the manufacturer’s instructions, and cloned as XbaI-SmaI (UP rmp ), SmaI (eryR) and SmaI-XhoI (DOWN rmp ) into a pBluescript vector digested with XbaI-XhoI (NEB). .. Amplification of upstream and downstream regions of the lgtF gene was performed with the primer couples lgtF-UP-fw / rv and lgtF-DO-fw / rv , respectively, using 50 ng of genomic DNA as template; the amplification of the chloramphenicol resistance gene ( cmR ) was done with the primer couple cloKOF / R using 10 ng of a synthetic DNA template (Geneart).



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    Image Search Results


    Journal: bioRxiv

    Article Title: CANVAS causing AAGGG repeat expansions cause tissue-specific reduction in RFC1 expression and increase sensitivity to DNA damage

    doi: 10.1101/2025.11.18.688292

    Figure Lengend Snippet:

    Article Snippet: Briefly, Firefly Luciferase plasmid and the repeat containing pBluescript vectors were digested with XbaI and NotI restriction enzymes (NEB), while pGint plasmid and pBluescript vectors were digested with NotI and BamHI restriction enzymes (NEB) at 37°C for 1 hour.

    Techniques: Luciferase